software sas version 8.1 Search Results


vero cells  (ATCC)
99
ATCC vero cells
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero cells - by Bioz Stars, 2026-04
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collagenase iv worthington biochemical ls004188 dnase i worthington biochemical ls006342 dulbecco  (Worthington Biochemical)
99
Worthington Biochemical collagenase iv worthington biochemical ls004188 dnase i worthington biochemical ls006342 dulbecco
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Collagenase Iv Worthington Biochemical Ls004188 Dnase I Worthington Biochemical Ls006342 Dulbecco, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagenase iv worthington biochemical ls004188 dnase i worthington biochemical ls006342 dulbecco - by Bioz Stars, 2026-04
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sas studio software  (SAS institute)
90
SAS institute sas studio software
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Sas Studio Software, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sas studio software - by Bioz Stars, 2026-04
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sas ondemand for academics software  (On Demand Pharmaceuticals)
90
On Demand Pharmaceuticals sas ondemand for academics software
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Sas Ondemand For Academics Software, supplied by On Demand Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sas ondemand for academics software - by Bioz Stars, 2026-04
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r version 2 81  (STATA Corporation)
99
STATA Corporation r version 2 81
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
R Version 2 81, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r version 2 81 - by Bioz Stars, 2026-04
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sas software 3.81  (SAS institute)
90
SAS institute sas software 3.81
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Sas Software 3.81, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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software studio 3.81  (SAS institute)
90
SAS institute software studio 3.81
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Software Studio 3.81, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software studio 3.81/product/SAS institute
Average 90 stars, based on 1 article reviews
software studio 3.81 - by Bioz Stars, 2026-04
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mixed linear model procedure of sas® studio software, lts 3.81  (On Demand Pharmaceuticals)
90
On Demand Pharmaceuticals mixed linear model procedure of sas® studio software, lts 3.81
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Mixed Linear Model Procedure Of Sas® Studio Software, Lts 3.81, supplied by On Demand Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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statistical analysis system (sas) software version 9.4  (SAS institute)
90
SAS institute statistical analysis system (sas) software version 9.4
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Statistical Analysis System (Sas) Software Version 9.4, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/statistical analysis system (sas) software version 9.4/product/SAS institute
Average 90 stars, based on 1 article reviews
statistical analysis system (sas) software version 9.4 - by Bioz Stars, 2026-04
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ondemand academics, version 3.81 software  (SAS institute)
90
SAS institute ondemand academics, version 3.81 software
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Ondemand Academics, Version 3.81 Software, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ondemand academics, version 3.81 software - by Bioz Stars, 2026-04
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sas proc glimmix  (SAS institute)
90
SAS institute sas proc glimmix
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Sas Proc Glimmix, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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version 12 0  (STATA Corporation)
99
STATA Corporation version 12 0
Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, <t>Vero,</t> <t>Huh7.5.1,</t> HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.
Version 12 0, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/version 12 0/product/STATA Corporation
Average 99 stars, based on 1 article reviews
version 12 0 - by Bioz Stars, 2026-04
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Image Search Results


Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, Vero, Huh7.5.1, HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.

Journal: Journal of Virology

Article Title: SFTSV utilizes AXL/GAS6 for entry via PI3K-PLC-dependent macropinocytosis activated by AXL-kinase

doi: 10.1128/jvi.00221-25

Figure Lengend Snippet: Genetic screening for cellular proteins involved in SFTSV entry. ( A ) Cell surface expression of DC-SIGN in HEK293, Vero, Huh7.5.1, HUVEC, and HEK293 DC-SIGN cells. HEK293 DC-SIGN : HEK293 cells expressing DC-SIGN are used as positive control. ( B ) SFTSV infection (MOI: 0.01) in various cells. Total cellular RNA was extracted at 24 hpi, and relative SFTSV RNA levels compared to those of HEK293 cells were measured using RT-qPCR. Data shown are mean + SD of three independent experiments. Significance in panel E was determined by t -test; NS, not significant; *** P < 0.001; **** P < 0.0001. ( C ) Scheme depicting genome-wide CRISPR activation screening for potential receptors involved in SFTSVpv infection. In brief, reporter cell populations were generated by infecting HEK293 cells stably integrated with the doxycycline-inducible dCas9-VPR gene with a CRISPRa-v2 lentiviral pooled library. Among these reporter cell populations, cells more susceptible to SFTSVpv bearing fluorescent protein reporter genes were sorted based on reporter protein expression upon infection. Unsorted cells infected with SFTSVpv were used as control cell populations. After three rounds of sorting, genomic DNAs were extracted from sorted and unsorted cells, and the integrated sgRNAs were analyzed by deep sequencing and MAGeCK software. ( D ) Confirmation of enrichment of cells susceptible to SFTSVpv after two rounds of sorting. Cells before sorting and those after first and second sortings were infected with SFTSVpv harboring crimson reporter gene at an MOI of 0.01, and expression of crimson was analyzed using flow cytometry at 72 hpi. ( E ) P -values were calculated from the three sorting rounds and unsorted groups by a negative binomial model using a modified distribution of RRA algorithm named alpha-RRA. Of the top 30 candidates, several membrane protein genes are shown.

Article Snippet: HEK293 and HEK293T cells (American Type Culture Collection; ATCC CRL-1573 and CRL-3216, respectively), Huh7.5.1 cells (human hepatocellular carcinoma; RRID CVCL-E049), Vero cells (monkey kidney; ATCC CRL-81), and their derivatives were cultured in DMEM with high glucose (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS (Biowest, Bradenton, FL, USA) and 1% penicillin-streptomycin mixed solution (Nacalai Tesque).

Techniques: Expressing, Positive Control, Infection, Quantitative RT-PCR, Genome Wide, CRISPR, Activation Assay, Generated, Stable Transfection, Control, Sequencing, Software, Flow Cytometry, Modification, Membrane